Objective: The aim of this study is to investigate the efficacy of concentrated growth factor (CGF) and hyaluronic acid (HA) application on the healing of acute tympanic membrane (TM) perforation.

Methods: A total of 30 male albino rats were included in the study. The animals were randomly divided into three groups with 9 rats in each group (A, B, C). A CGF was applied to the perforated TMs of rats in group A. A gelfoam particle soaked in 1% hyaluronan was applied to the perforations in group B. The rats in group C were left to heal spontaneously. All rats were kept under control for 21 days. TM healing was evaluated. On the 21st day, all rats were decapitated for histopathological evaluation.

Results: In group A, 18 of the 18 TM perforations were closed and the mean healing time was 12.11 days. In group B, 18 of the 18 TM perforations were closed and the mean healing time was 15.05 days. In group C, 16 of 18 TM perforations were closed and the mean closure time was 17.11 days. There was a statistically significant difference in the mean TM closure time in all groups at the end of the 21st day (p=0.001).

Conclusion: In this study, we used autologous CGF and HA in the treatment of acute TM perforations in rats. We observed that the application of both products was a more effective method than the control group. We think that using these methods, which are easy, inexpensive, and have low patient morbidity, as graft or graft support in chronic TM perforations will be beneficial and successful results will increase.