The Sequencing of the Insulin-like Growth Factor 1 and Fibulin 5 Gene Variants in the Pre and Post-menopausal Women with Stress Urinary Incontinence
1University of Health Sciences, Hamidiye Faculty of Medicine, Department of Medical Biochemistry, İstanbul, Turkey
2İstanbul University, Aziz Sancar Institute of Experimental Medicine (ASDETAE), Department of Molecular Medicine, İstanbul, Turkey
3İstanbul University Cerrahpaşa Faculty of Medicine, Department of Urology, İstanbul, Turkey
Eur Arch Med Res 2019; 35(3): 182-185 DOI: 10.4274/eamr.galenos.2019.06977
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Abstract

Objective: Urinary incontinence (UI) is defined as a social problem via involuntary incontinence. Genetic variations that occur especially in muscle and connective tissue can cause the susceptibility stress type UI. We aimed to investigate the variations in insulin-like growth factor 1 and Fibulin 5 (FBLN5) genes in the pre and post-menopausal women with stress UI (SUI).

Methods: The study consisted of 4 groups: 43 premenopausal women with SUI, 30 premenopausal women without SUI, 43 postmenopausal women with SUI and 30 postmenopausal women without SUI. DNA was isolated from blood samples and sequenced with Illumina®MiSeq. The results were analyzed with SPSS22 (IBM Corp., Armonk, NY, USA) and p value less than 0.05 was considered as statistically significant.

Results: The A>G variant of rs6214 was found 5.26% (2/38) in the patient group and 0% (0/30) in the control group of the premenopausal group (p>0.05). This variant was found 2.44% (1/41) in the postmenopausal SUI group (p>0.05). The FBLN5 rs929608 variant was not found in any group.

Conclusion: No significant association was found between UI and these variants.